christian stehlik (Addgene inc)
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christian stehlik
Christian Stehlik, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/christian+stehlik/pEGFP-C2-NLRP3+(Plasmid+%2373955)/10__1038_slash_s44318___026___00755___7-322-6-8
Average 93 stars, based on 31 article reviews
Christian Stehlik, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/christian+stehlik/pEGFP-C2-NLRP3+(Plasmid+%2373955)/10__1038_slash_s44318___026___00755___7-322-6-8
Average 93 stars, based on 31 article reviews
christian stehlik - by Bioz Stars,
2026-09
93/100 stars
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Transfection:Article Title: Copper overload worsens the inflammatory response of microglia to amyloid beta (Aβ) by impairing phagocytosis and promoting mitochondrial DNA-mediated NLRP3 inflammasome activation. Article Snippet: After triple washing, they were incubated with 20 μg/ml GST-PFO in antibody diluent buffer (Agilent Technologies, S302283-2) for 45 min before immunostaining. .. To evaluate the presence of apoptosis-associated speck-like protein containing a CARD (ASC) oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, which was generously provided by Article Title: Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis. Article Snippet: .. To evaluate the presence of ASC oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, a gift from Article Title: Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis Article Snippet: .. To evaluate the presence of ASC oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, a gift from Article Title: Copper overload worsens the inflammatory response of microglia to amyloid beta (Aβ) by impairing phagocytosis and promoting mitochondrial DNA-mediated NLRP3 inflammasome activation Article Snippet: After triple washing, they were incubated with 20 μg/ml GST-PFO in antibody diluent buffer (Agilent Technologies, S302283-2) for 45 min before immunostaining. .. To evaluate the presence of apoptosis-associated speck-like protein containing a CARD (ASC) oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, which was generously provided by Plasmid Preparation:Article Title: Copper overload worsens the inflammatory response of microglia to amyloid beta (Aβ) by impairing phagocytosis and promoting mitochondrial DNA-mediated NLRP3 inflammasome activation. Article Snippet: After triple washing, they were incubated with 20 μg/ml GST-PFO in antibody diluent buffer (Agilent Technologies, S302283-2) for 45 min before immunostaining. .. To evaluate the presence of apoptosis-associated speck-like protein containing a CARD (ASC) oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, which was generously provided by Article Title: Nitroxoline is a novel inhibitor of NLRP3-dependent pyroptosis Article Snippet: Cells were analyzed using BD FACSLyric flow cytometer (BD Biosciences, NJ, USA). .. pcDNA3-Myc-ASC and pEGFP-C2-NLRP3 were a gift from Article Title: GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome priming Article Snippet: Cloning of Flag NLRP3 full-length and NLRP3 deletion mutants Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific) was used for amplification of NLRP3 gene cDNA using the pEGFPC2-NLRP3 plasmid as template for making the Flag-NLRP3 variants. .. The pEGFP-C2-NLRP3 was a gift from Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination. Article Snippet: .. Article Title: Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis. Article Snippet: .. To evaluate the presence of ASC oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, a gift from Article Title: Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis Article Snippet: .. To evaluate the presence of ASC oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, a gift from Article Title: Copper overload worsens the inflammatory response of microglia to amyloid beta (Aβ) by impairing phagocytosis and promoting mitochondrial DNA-mediated NLRP3 inflammasome activation Article Snippet: After triple washing, they were incubated with 20 μg/ml GST-PFO in antibody diluent buffer (Agilent Technologies, S302283-2) for 45 min before immunostaining. .. To evaluate the presence of apoptosis-associated speck-like protein containing a CARD (ASC) oligomers, cells were transfected with the pLEX-MSC-ASC-GFP plasmid, which was generously provided by other:Article Title: Nitroxoline is a novel inhibitor of NLRP3-dependent pyroptosis. Article Snippet: Cells were analyzed using BD FACSLyric flow cytometer (BD Biosciences, NJ, USA). |